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Category:Video games developed in Poland Category:Windows games Category:Windows-only games Category:Windows-only freeware Category:Windows-only commercial software Category:Card games introduced in 2004 Category:Card game video gamesNovel topological classification of malignant pleural mesothelioma using DNA flow cytometry, chromosome banding, and immunohistochemistry. Despite advances in the understanding of its pathology, malignant pleural mesothelioma (MPM) continues to be a difficult tumor to subtype. In the present study, we used multiple variables to further characterize the cytogenetic and immunophenotypic features of MPM. Thirty-three pleural mesotheliomas were analyzed for DNA content and ploidy using flow cytometry. For comparison, results were correlated with histomorphology, immunohistochemical staining, and in vitro cell growth. Twenty-eight of 33 (85%) pleural mesotheliomas showed one or more of the following features: diploid, hyperdiploid, tetraploid, hypodiploid, S-phase, or a combination of these. One tumor had a normal DNA content. The remaining five tumors (15%) showed no evidence of aneuploidy, but may have contained one or more populations of abnormal cells. Chromosome analysis revealed a near-diploid karyotype in 13 tumors (40%); the remaining 20 tumors (60%) had a near-tetraploid karyotype. Tumors with a near-diploid or near-tetraploid karyotype showed much less cellular differentiation and a significantly worse prognosis than tumors with a near-diploid karyotype, but with normal ploidy content (P = 0.0047). Several other chromosomal abnormalities, including tetrasomy, rearrangement of chromosomes 1 or 9, and deletion of 1p, were also noted. Sixty per cent (9 of 15) of tumors with a near-tetraploid karyotype were also positive for MOC-31, an epithelial membrane antigen marker. Twelve of 19 (63%) of the malignant tumors were positive for vimentin, and 14 of 19 (74%) were positive for pancytokeratin. By contrast, 29% (5 of 18) of the benign tumors were positive for MOC-31, 11% (2 of 18)


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